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Ex vivo culture-expansion of ALDH br atrial cells (a). Photomicrographs of ALDH br cells cultured for 7 and 12 days in <t>MesenCult</t> medium. ALDH dim⁡ cells were dead by day 7 (b). Growth curves of cells in different growth media. Cells were cultured in MesenCult medium up to P10 (arrow). From P11 on, MesenCult was either maintained or replaced by RPMI/FCS. Symbols (squares and diamonds) indicate cell passage (no data available from P1 to P7; dotted line) (c). AlamarBlue assay on cells (P40) cultured in either MesenCult or RPMI/FCS (AR%: percent AlamarBlue reduction) (d). Dose-response study of Imatinib with respect to growth inhibition of expanded ALDH br cells (P25).
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Ex vivo culture-expansion of ALDH br atrial cells (a). Photomicrographs of ALDH br cells cultured for 7 and 12 days in <t>MesenCult</t> medium. ALDH dim⁡ cells were dead by day 7 (b). Growth curves of cells in different growth media. Cells were cultured in MesenCult medium up to P10 (arrow). From P11 on, MesenCult was either maintained or replaced by RPMI/FCS. Symbols (squares and diamonds) indicate cell passage (no data available from P1 to P7; dotted line) (c). AlamarBlue assay on cells (P40) cultured in either MesenCult or RPMI/FCS (AR%: percent AlamarBlue reduction) (d). Dose-response study of Imatinib with respect to growth inhibition of expanded ALDH br cells (P25).
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Ex vivo culture-expansion of ALDH br atrial cells (a). Photomicrographs of ALDH br cells cultured for 7 and 12 days in <t>MesenCult</t> medium. ALDH dim⁡ cells were dead by day 7 (b). Growth curves of cells in different growth media. Cells were cultured in MesenCult medium up to P10 (arrow). From P11 on, MesenCult was either maintained or replaced by RPMI/FCS. Symbols (squares and diamonds) indicate cell passage (no data available from P1 to P7; dotted line) (c). AlamarBlue assay on cells (P40) cultured in either MesenCult or RPMI/FCS (AR%: percent AlamarBlue reduction) (d). Dose-response study of Imatinib with respect to growth inhibition of expanded ALDH br cells (P25).
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Ex vivo culture-expansion of ALDH br atrial cells (a). Photomicrographs of ALDH br cells cultured for 7 and 12 days in <t>MesenCult</t> medium. ALDH dim⁡ cells were dead by day 7 (b). Growth curves of cells in different growth media. Cells were cultured in MesenCult medium up to P10 (arrow). From P11 on, MesenCult was either maintained or replaced by RPMI/FCS. Symbols (squares and diamonds) indicate cell passage (no data available from P1 to P7; dotted line) (c). AlamarBlue assay on cells (P40) cultured in either MesenCult or RPMI/FCS (AR%: percent AlamarBlue reduction) (d). Dose-response study of Imatinib with respect to growth inhibition of expanded ALDH br cells (P25).
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Ex vivo culture-expansion of ALDH br atrial cells (a). Photomicrographs of ALDH br cells cultured for 7 and 12 days in MesenCult medium. ALDH dim⁡ cells were dead by day 7 (b). Growth curves of cells in different growth media. Cells were cultured in MesenCult medium up to P10 (arrow). From P11 on, MesenCult was either maintained or replaced by RPMI/FCS. Symbols (squares and diamonds) indicate cell passage (no data available from P1 to P7; dotted line) (c). AlamarBlue assay on cells (P40) cultured in either MesenCult or RPMI/FCS (AR%: percent AlamarBlue reduction) (d). Dose-response study of Imatinib with respect to growth inhibition of expanded ALDH br cells (P25).

Journal: BioMed Research International

Article Title: Characterization of Cardiac-Resident Progenitor Cells Expressing High Aldehyde Dehydrogenase Activity

doi: 10.1155/2013/503047

Figure Lengend Snippet: Ex vivo culture-expansion of ALDH br atrial cells (a). Photomicrographs of ALDH br cells cultured for 7 and 12 days in MesenCult medium. ALDH dim⁡ cells were dead by day 7 (b). Growth curves of cells in different growth media. Cells were cultured in MesenCult medium up to P10 (arrow). From P11 on, MesenCult was either maintained or replaced by RPMI/FCS. Symbols (squares and diamonds) indicate cell passage (no data available from P1 to P7; dotted line) (c). AlamarBlue assay on cells (P40) cultured in either MesenCult or RPMI/FCS (AR%: percent AlamarBlue reduction) (d). Dose-response study of Imatinib with respect to growth inhibition of expanded ALDH br cells (P25).

Article Snippet: ALDH br cells were cultured in MesenCult medium (MesenCult MSC Basal Medium supplemented with serum-containing MesenCult MSC Stimulatory Supplements-Mouse; STEMCELL Technologies).

Techniques: Ex Vivo, Cell Culture, Alamar Blue Assay, Inhibition

(a)/(b) Flow cytometric analysis of ALDH br atrial cells expanded in MesenCult (P11) or RPMI/FCS medium (P13). (c) Percentages of cells (mean, SD) expressing the indicated markers (data from 3 separate experiments in MesenCult). (d) Two-color analysis of pericyte/perivascular markers (NG2, CD146, and CD140b; numbers are cell percentages). (e) c-kit immunostaining of expanded ALDH br cells. Nuclear staining with Hoechst 33342 (CTRL, secondary Ab only as a control).

Journal: BioMed Research International

Article Title: Characterization of Cardiac-Resident Progenitor Cells Expressing High Aldehyde Dehydrogenase Activity

doi: 10.1155/2013/503047

Figure Lengend Snippet: (a)/(b) Flow cytometric analysis of ALDH br atrial cells expanded in MesenCult (P11) or RPMI/FCS medium (P13). (c) Percentages of cells (mean, SD) expressing the indicated markers (data from 3 separate experiments in MesenCult). (d) Two-color analysis of pericyte/perivascular markers (NG2, CD146, and CD140b; numbers are cell percentages). (e) c-kit immunostaining of expanded ALDH br cells. Nuclear staining with Hoechst 33342 (CTRL, secondary Ab only as a control).

Article Snippet: ALDH br cells were cultured in MesenCult medium (MesenCult MSC Basal Medium supplemented with serum-containing MesenCult MSC Stimulatory Supplements-Mouse; STEMCELL Technologies).

Techniques: Expressing, Immunostaining, Staining, Control